What is the main purpose of Orange G in a Pap stain?

The primary purpose of Orange G in a Pap stain is to stain keratin an intense orange color. In Pap smears, which are used for cervical cancer screening, the staining process involves several reagents that help differentiate various cellular components based on their properties. Orange G specifically targets keratinized cells, enabling pathologists to identify abnormal cell types effectively. This intense coloration of keratin helps clinicians assess the presence of dysplastic or neoplastic changes in the cervical epithelium, providing valuable information for diagnosis and management.

While other options suggest different staining purposes, they do not align with the specific role that Orange G plays in the context of a Pap smear.