What is the last step in the Pap stain preparation process?

The final step in the Pap stain preparation process involves mounting the slides using a medium, which is critical for preserving the stained specimen for microscopic examination. This step allows for the careful placement of a coverslip over the stained cells, which protects the specimen and ensures that it can be properly viewed under a microscope. The mounting medium helps to maintain the clarity of the stain and provides an environment that minimizes air exposure and potential degradation of the sample.

Mounting plays a vital role in facilitating the examination and interpretation of the cellular morphology that is essential for diagnosing any pathological conditions. By using a suitable mounting medium, the quality and longevity of the prepared slides are enhanced, ensuring that the results remain reliable during analysis.