What is the first step in the Pap stain procedure?

The first step in the Pap stain procedure is the removal of the fixative. This step is critical because the fixative, such as 95% ethanol, is used to preserve the cellular morphology and structure of the samples obtained from the cervix. Before applying the staining agents, the slides must be treated to extract the fixative. This prepares the cell samples for subsequent steps in the staining process, ensuring that the cellular components can be effectively stained and visualized.

This foundational step is important for achieving clear and accurate results in the cytological evaluation of the prepared slides. Properly removing the fixative allows for better penetration of the stains in the following steps, facilitating optimal nuclear and cytoplasmic staining.