What effect does Blueing have on stained tissue?

Blueing refers to a process in histological staining where the stained tissue is treated to shift its color, typically from red-brown to a blue-purple hue. This transformation is primarily relevant when using stains like Hematoxylin and Eosin (H&E), where the Hematoxylin provides a blue coloration to the nuclei. When blueing agents such as ammonia or other alkaline solutions are applied, they can enhance this blue color, providing better contrast against other tissue components.

This process is essential for improving the visualization of cellular details under a microscope. By altering the color in this way, pathologists and laboratory technicians can more easily discern different structures and components in the tissue sample, aiding in diagnosis and analysis. Thus, the conversion from red-brown to blue-purple is a significant effect of blueing on stained tissue.