During the Cell Block Prep for sputum, what is the primary fixative used?

The primary fixative used during the Cell Block Prep for sputum is Formalin. Formalin is a solution of formaldehyde in water, commonly used in histopathology as a tissue fixative because it preserves cellular morphology and prevents autolysis and decomposition of tissue samples. It cross-links proteins and nucleic acids, which is essential in maintaining the integrity of the cellular structures being examined.

In the context of sputum specimens, which are often used to diagnose pulmonary conditions, the use of Formalin facilitates the accurate evaluation of cells and allows pathologists to obtain clear and interpretable results.

While Bouin's fixative, which is typically used for soft and delicate tissues, is effective for some types of samples, it is not typically the primary choice for cell block preparations in respiratory samples like sputum, where Formalin is more appropriate for ensuring preservation and cellular structure.